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Title   REtroviral Vector를 이용한 인체 간암세포주로의 Interleukin-2 유전자 형질도입의 효과 ( Effects of Interleukin-2 Transduction into the Human Hepatoma Cell Lines Using Retroviral Vector )
Publicationinfo   1997 Jan; 029(04): 555-565.
Key_word   Interleukin-2, Hepatoma cell line, Tumor regression
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Abstract   Purpose: We compared the differences between parent hepatoma cell lines and interleukin-2(IL-2) transduced hepatoma cell lines using N2A/IL-2 and LNC/IL-2 retrovirus with regards to in vitro sensitivity to peripheral blood monocytes and in vivo tumorigenic activity. Materials and Methods: Retroviral vector and producer cell line were constructed and IL-2 gene was transduced into the human hepatoma cell lines(SK-Hepl, Hep-G2, Hep-3B). IL-2 secretion after IL-2 transduction was measured by ELISA. MTT assay for in vitro sensitivity to peripheral blood monocytes was performed and the tumorigenic activity was observed in BALB/c mice and nude mice. Results: IL-2 secretion was 186 pg/10 6cells/24 hrs in SK-Hep1 cell line and was 147 pg/10 6 cells/24 hrs in Hep-3B cell line with N2A/IL-2 retroviral vector and was 55,000 pg/10 6 cells/24 hrs with LNC/IL-2 retroviral vector. In vitro sensitivity to peripheral blood monocytes was increased by 163.8-254% in IL-2 transduced hepatoma cell lines(Hep -3B/N2A/IL-2, Hep-G2/N2A/IL-2) compared to those of the parent cell lines. The tumorigenicity was observed in 1 of 3 BALB/c mice and all 3 nude mice. Simultaneous injection of 1 * 10 7cells of the parent cell line(Hep-3B) into the right flank and IL-2 transduced cell line(Hep-3B/LNC/IL-2) into the left flank of the three BALB/c mice and of 5 * 10 5cells for the three nude mice resulted in a complete regression of the IL-2 modified tumor cell line(Hep-3B/LNC/IL-2) in 3 weeks and the parent cell line(Hep-3B) in 5 weeks. But, after the injection of 1.5 * 10 7cells for other five nude mice, the tumor of the IL-2 transduced hepatoma cell line(Hep-3B/LNC/IL-2) was gradually disappeared, and the tumor of the parent hepatoma cell line(Hep-3B) was initially decreased and then gradually regrew 20 days later. Conclusion: IL-2 transduced hepatoma cell lines secreting IL-2 became more sensitive to peripheral blood monocytes and resulted in the increased antigenicity to the tumors formed by IL-2 transduced hepatoma cell line and parent cell line, and finally resulted in the regression of the tumors in experimental animals.
저 자   공수정(Soo Jung Gong),유내춘(Nae Choon Yoo),김주항(Joo Hang Kim),신동환(Dong Hwan Shin),엄효동(Hyo Dong Uhm),정숙정(Sook Jung Jung),조재용(Jae Yong Cho),라선영(Seon Young Rah),김연수(Yeon Soo Kim),정현철(Hyun Chul Chung),노재경(Jae Kyung Roh),민진식(Jin Sik Min),김병수(Byung Soo Kim),